Journal: bioRxiv
Article Title: The uptake of metallic nanoparticles in breast cancer cell lines is modulated by the HA-CD44 axis
doi: 10.1101/2025.02.12.637873
Figure Lengend Snippet: HAS2 expression, and effect of HA coat modulation on NP uptake A. HAS2 expression in 4 different breast cancer cell lines measured by qRT-PCR. Relative quantification was calculated with HMLE-E sample as a reference. Measures of expression were performed in triplicates, and error bars represent RQ min and RQ max values. B. HAS3 expression in 4 different breast cancer cell lines, measured by qRT-PCR. Relative quantification was calculated with HMLE-M sample as a reference. Measures of expression were performed in triplicates, and error bars represent RQ min and RQ max values. For A and B, t he statistical significance was determined by using the one-way Anova test, with a Sidak’s multiple comparison test. Note: ns, non-significant; * p < 0.05, ** p <0.005 and **** p < 0.0001. C. Representative images of hyaluronic acid staining in the 4 different cell lines. Nuclei were stained with Hoescht (blue) and HA Binding proteins coupled to biotin were further detected using streptavidin-FITC (green). Images were obtained by confocal microscopy. Scale bar is 10µm. D. Mean Fluorescence Intensity of HABP from at least 10000 cells for each of the 4 indicated cell lines, analysed by flow cytometry. Analysis was performed in triplicates. E. SSC-A deviation signals from T47D and MDA-MB231 cells treated with hyaluronidase and exposed to NM100 or NM103 TiO2 NPs. The graph represents the mean of data from 10000 cells, and in duplicates. The statistical analysis comparing untreated vs treated cells was performed with one-way anova. Ns=non significant, *indicates a p-value<0,05 and **indicates a p-value<0,01.
Article Snippet: The references of the Taqman probe sets used are as follows : GAPDH : Hs99999905_m1, CD44v2-10 : Hs01075866_m1, CD44s : Hs01081473_m1, HAS2 : Hs00193435_m1, HAS3 : Hs00193436_m1.
Techniques: Expressing, Quantitative RT-PCR, Quantitative Proteomics, Comparison, Staining, Binding Assay, Confocal Microscopy, Fluorescence, Flow Cytometry
Santa Cruz Biotechnology is a verified supplier 
